Livros e Publicações
URI permanente para esta coleçãohttps://bdc.icmbio.gov.br/handle/cecav/1487
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Resultados da Pesquisa
- Prevalence of internal tumor in Green Turtles (Chelonia mydas) affected by Fibropapilomatosis in Brazil(2015) D´AZEREDO, F; PIRES, T; MONTE, T; MELO, T.D; VELLOSO, R; GOLDBERG, D; BAPTISTOTTE, C; ALMOSNY, N; BRUNO, S
Item Deeper mitochondrial sequencing reveals cryptic diversity and structure in Brazilian green turtle rookeries.(Chelonian Conservation and Biology, 2015) SHAMBLIN, B. M; DUTTON, P. H; BJORNDAL, K. A; BOLTEN, A. B; NARO-MACIEL, E; SANTOS, A. J. B; BELLINI, C; BAPTISTOTTE, C; MARCOVALDI, M. A; NAIRN, C. JGenetic markers are often used to designate population units for management and conservation, but widespread sharing of mitochondrial DNA control-region haplotypes defined from short (, 500 base-pair [bp]) sequences often limits inferences of population connectivity in marine turtles. Haplotype CM-A8, defined from 490-bp sequences, dominated the haplotype profiles of the 3 major green turtle (Chelonia mydas) rookeries in Brazil. Previous analyses based on 490-bp haplotypes did not detect differentiation between the northern rookeries of Atol das Rocas and Fernando de Norohna, but did indicate differentiation of the northern rookeries from Trindade Island in the south. We reexamined the stock structure of the Brazilian green turtle rookeries using 817-bp control region and mitochondrial short tandem repeat (mtSTR) sequences. Nine 490-bp haplotypes were subdivided into 41 haplotypes by combining 817-bp and mtSTR sequences. Eight of the 14 CM-A8 turtles from Fernando de Noronha carried mtSTR haplotypes that were not detected in the larger rookeries. Pairwise exact tests indicated that the northern Brazilian green turtle rookeries of the Rocas Atoll and Fernando de Noronha are discrete populations with respect to female natal homing. Moreover, several apparently endemic markers in the 3 Brazilian green turtle nesting populations should improve resolution of future mixedstock analyses. Comparable data are needed from green turtle rookeries in the central and eastern Atlantic to assess structure and connectivity at the ocean basin scale.- The Interplay of Homing and Dispersal in Green Turtles: A Focus on the Southwestern Atlantic(Journal of Heredity, 2012-10-08) NARO-MACIEL, E; BONDIOLI, A.C.V; MARTIN, M; ALMEIDA, A.P; BAPTISTOTTE, C; BELLINI, C; MARCOVALDI, M.A; SANTOS, A.J.B; AMATO, GCurrent understanding of spatial ecology is insufficient in many threatened marine species, failing to provide a solid basis for conservation and management. To address this issue for globally endangered green turtles, we investigated their population distribution by sequencing a mitochondrial control region segment from the Rocas Atoll courtship area (n = 30 males) and four feeding grounds (FGs) in Brazil (n = 397), and compared our findings to published data (nnesting = 1205; nfeeding = 1587). At Rocas Atoll, the first Atlantic courtship area sequenced to date, we found males were differentiated from local juveniles but not from nesting females. In combination with tag data, this indicates possible male philopatry. The most common haplotypes detected at the study sites were CMA-08 and CMA-05, and significant temporal variation was not revealed. Although feeding grounds were differentiated overall, intra-regional structure was less pronounced. Ascension was the primary natal source of the study FGs, with Surinam and Trindade as secondary sources. The study clarified the primary connectivity between Trindade and Brazil. Possible linkages to African populations were considered, but there was insufficient resolution to conclusively determine this connection. The distribution of FG haplotype lineages was nonrandom and indicative of regional clustering. The study investigated impacts of population size, geographic distance, ocean currents, and juvenile natal homing on connectivity, addressed calls for increased genetic sampling in the southwestern Atlantic, and provided data important for conservation of globally endangered green turtles.
- Establecimiento de escores para determinar el grado de severidad de la fibropapilomatosis en Chelonia mydas (Testudines, Cheloniidae) capturadas en la costa brasilera(2012) ROSSI, S; SÁNCHEZ-SARMIENTO, A.M; VANSTREEL, R.E.T; SANTOS, R.G; BAPTISTOTTE, C; LIMA, E.H.S.M; BECKER, J.H; MATUSHIMA, E.RLa fibropapilomatosis (FP) es una enfermedad que afecta tortugas marinas, en especial a la especie Chelonia mydas, siendo caracterizada por tumores cutáneos benignos. Es considerada una de las principales amenazas para su conservación junto a la captura por pesca y degradación del hábitat. Existe un consenso de que la FP tendría múltiples causas, incluyendo un agente viral, factores genéticos y ambientales. Fueron estudiados especímenes de C. mydas provenientes de UbatubaSP, Vitória-ES y Almofala-CE, áreas de alimentación en Brasil. Se registraron datos biométricos: Largo curvo del caparazón (cm), Ancho curvo del caparazón (cm) y Masa corporal (kg), tamaño y cantidad de tumores por región anatómica, conforme protocolo del Proyecto Tamar-ICMBio, clasificando los tumores por categorías: 1 (< 1 cm), 2 (1 cm ≤ tumor < 4 cm), 3 (4 cm ≤ tumor < 10 cm) y 4 (≥ 10 cm). El objetivo de este trabajo fue utilizar la cantidad de tumores por categoría para definir escores de gravedad de la enfermedad. Posteriormente esos escores serán correlacionados al índice de condición corporal (ICC), análisis de organoclorados y de función celular. La nueva propuesta, aún en desarrollo, contempla aplicación de modelaje estadístico utilizando estas categorías y la aplicación de una puntuación por categoría. Por ejemplo, categorías 1 y 2 llevarían menor puntuación por clasificar tumores más pequeños. Tal propuesta será utilizada para C. mydas de la costa brasilera, aplicándose mejor a esas poblaciones y los escores posibilitarán una mejor evaluación de la severidad de la FP (leve, moderada, grave, muy grave).
- Distribución anatómica de fibropapilomas en tortugas verdes, Chelonia mydas (Testudines, Cheloniidae), capturadas en la costa brasilera(2012) SÁNCHEZ-SARMIENTO, A.M; ROSSI, S; VANSTREEL, R.E.T; SANTOS, R.G; BAPTISTOTTE, C; BECKER, J.H; LIMA, E.H.S.M; MATUSHIMA, E.RLas tortugas marinas se encuentran mundial y regionalmente amenazadas de extinción. La tortuga verde, Chelonia mydas, está clasificada como “Amenazada” por la Unión Internacional para la Conservación de la Naturaleza y es listada como vulnerable de extinción por el Instituto Brasilero de Medio Ambiente y de los Recursos Naturales Renovables. Las amenazas para la especie incluyen captura por pesca, degradación del hábitat en las áreas de alimentación y anidación, polución marina y enfermedades. Una de las principales amenazas para la conservación de esta especie es la fibropapilomatosis, Green turtle fibropapilloma disease, una enfermedad tumoral que se destaca por su carácter multifactorial, amplia distribución e impacto. Se caracterizaron los fibropapilomas de 88 individuos provenientes de Almofala-CE (n=4), Ubatuba-SP (n=61) y Vitória-ES (n=23). En total, 3035 fibropapilomas (34,49 ± 27,69), de 1 a 129 por tortuga, fueron clasificados por categorías de tamaño: (1) < 1 cm (42,9% de los tumores), (2) 1 ≤ tumor < 4 cm (50,71%), (3) 4 ≤ tumor < 10 cm (6,06%), (4) ≥ 10 cm (0,33%). Se constató que los tumores se distribuyen en mayor cantidad en las aletas anteriores y posteriores (incluyendo en ambas piel adyacente) con un 44,18% y 29,16% respectivamente, región cervical (11,63%), plastrón (5,01%), ojos (4,15%), región inguinal y cola (3,23%), caparazón (1,75%) y escamas córneas de la cabeza (0,89%). Futuramente, los resultados serán correlacionados al histórico individual y concentraciones de compuestos organoclorados, estableciendo nuevas conductas para el análisis de la gravedad de esta enfermedad en tortugas verdes provenientes de la costa brasilera.
- Comparison between corporal subjetive classification and body condition index (BCI) for Chelonia mydas (Testudines, Cheloniidae) caught in Brazilian coast(2012) SÁNCHEZ-SARMIENTO, A.M; ROSSI, S; VANSTREEL, R.E.T; SANTOS, R.G; MARIGO, J; BERTOZZI, C.P; BAPTISTOTTE, C; BECKER, J.H; MATUSHIMA, E.RChelonia mydas, green sea turtle, is an endangered species by the IUCN (2012) and a vulnerable species in Brazil. The threats are catch, habitat degradation (including pollution) and diseases. The fibropapillomatosis (FP) is one of the greatest threats against the survival of C. mydas due to the multifactorial causes and impact all over the world. Biometric data (Curved Carapace Length-CCL, Curved Carapace WidthCCW and Body Mass-BM) of green sea turtles from Ubatuba-SP, Praia Grande-SP and Vitória-ES were recorded. Straight Carapace Length (SCL) was calculated from CCL and was used to obtain the Body Condition Index (BCI = BM / SCL3). BCI was tested versus subjective body condition (poor, fair and good), which evaluates the general aspect and concavity of plastron. The results demonstrated that the subjective classification is reliable for animals with poor condition; nevertheless, this classification is not clear for turtles estimated as fair and good. On the other hand, the objective classification (BCI) was an appropriate indicator for body condition. BCI of specimens caught by fishing net (1.54 ± 0.03) was higher than green sea turtles from rehabilitation (1.30 ± 0.08) or those found stranded or floating (1.23 ± 0.05). BCI was higher in females (1.45 ± 0.03) than males (1.28 ± 0.06). Further studies will attempt to correlate BCI with presence and intensity of FP and levels of organochlorine pollutants in tissues samples from C. mydas caught in other areas of Brazilian coast, aiming to clarify the role and impact of FP for green sea turtles conservation
Item Application of flow cytometry and gas chromatography to Study fibropapillomatosis in chelonia mydas (testudines, Cheloniidae) in brazil and their contributions for conservation of this species.(2012) ROSSI, S; SANCHEZ-SARMIENTO, A.M; VALE, L.A.S; GENOY-PUERTO, E. A; PRIOSTE, F.E.S; GATTAMORTA, M.A; KINOSHITA, D; HAZARBASSANOV, N.G.T.Q; BECKER, J.H; LIMA, E.H.S.M; BAPTISTOTTE, C; GOLDBERG, D.W; SANTOS, A.J.B; BORTOLON, L. F; MATUSHIMA, E. RItem Fibropapilomatose em Tartarugas Verdes (Chelonia mydas) da Bahia - Caracterização Molecular do chhv.(2011-11) RODENBUSCH, C. R; PIRES, T. T; BAPTISTOTTE, C; CANAL, C. WA fibropapilomatose (FP) é uma doença neoplásica caracterizada por um único ou múltiplos crescimentos fibroepiteliais cutáneos, com uma superficie verrugosa ou plana, e aparecendo como fibromas nas visceras (Kang et al. 2008). Os tumores se localizam ao redor dos olhos, na conjuntiva, cavidade oral, pescoço, nadadeiras, cauda, áreas axilares e inguinais e também em órgãos viscerais. O tamanho varia de poucos milimetros até 30 centimetros de diâmetro e os tumores podem causar a morte do animal por interferir na visão, locomoção, alimentação, respiração; já os tumores viscerais podem ser invasivos e prejudicar a função do órgão afetado (George 1997; Yu et al. 2000). O primeiro registro de FP na costa brasileira é de 1986. Entre 2000 e 2004, de 4.471 tartarugas-verdes (Chelonia mydas) examinadas 14,96% apresentavam tumores. A prevalência de tumores por ano foi de 12,91% (2000, n = 604), 14,96% (2001, n = 809), 14,79% (2002, n = 818), 19,95% (2003, n = 842) e 12,95% (2004, n = 1398) (Baptistotte et al. 2005). O padrão de disseminação da doença durante surtos em tartarugas-verdes de cativeiro consiste com uma etiologia contagiosa. Um herpesvirus (chelonid herpesvirus 5- ChHV 5) tem sido identificado em fibropapilomas (Herbst et al. 1995, Davison et al. 2009) e está presente em 100% dos tumores induzidos por inoculação de filtrados de células tumorais (Ene et al. 2005) e em 95% das infecções naturais, sendo que em 79% dos fibropapilomas e fibromas analisados por PCR em tempo-real quantitativo, o virus estava presente em niveis que excediam 10+ cópias por 100 ng de DNA total de tumores (Quackenbush et al. 2001). O presente estudo tem como objetivo detectar e quantificar o ChHV 5 em fibropapilomas de tartarugas- verdes do litoral da Bahia.- Coastal Habitat Degradation and Green Sea Turtle Diets in Southeastern Brazil.(Marine Pollution Bulletin, 2011-06) SANTOS, R. G; MARTINS, A. S; FARIAS, J. N; HORTA, P. A; PINHEIRO, H. T; TOREZANI, E; BAPTISTOTTE, C; SEMINOFF, J. A; BALAZS, G. H; WORK, T. MTo show the influence of coastal habitat degradation on the availability of food for green turtles (Chelonia mydas), we assessed the dietary preferences and macroalgae community at a feeding area in a highly urbanized region. The area showed low species richness and was classified as degraded. We examined stomach contents of 15 dead stranded turtles (CCL = 44.0 cm (SD 6.7 cm)). The diet was composed primarily of green algae Ulva spp. (83.6%). In contrast, the macroalgae community was dominated by the green alga Caulerpa mexicana. We found a selection for red algae, seagrass and Ulva spp., and avoidance for C. mexicana and brown alga Dictyopteris delicatula. The low diversity of available food items, possibly a result of environmental degradation, likely contributed to the low dietary diversity. The nutritional implications of this restricted diet are unclear.
Item Relationship between fibropapillomatosis and environmental quality: a case study with Chelonia mydas off Brazil.(2010-02-24) SANTOS, R. G; MARTINS, A. S; TOREZANI, E; BAPTISTOTTE, C; FARIAS, J. N; HORTA, P. A; WORK, T. M; BALAZS, G. HWe documented the presence of fibropapillomatosis (FP), a debilitating tumor-forming disease, in marine turtles in Espírito Santo Bay (Brazil) from March 2007 to April 2008, and assessed the value of a specific environmental index for predicting the prevalence of FP. Turtles were captured monthly with entanglement nets and scored for presence and severity of FP. For the assessment of habitat quality, we used the ecological evaluation index (EEI) based on benthic macrophytes. The FPfree control area was classified as good quality (EEI = 8) and the study area, with high FP prevalence, was classified as bad quality (EEI = 2). Prevalence of FP in the study area was 58.3% with an average of 40 tumors per individual, and prevalence varied positively with curved carapace length (CCL). No FP was seen in the control area. The number of turtles heavily afflicted (tumor score category 3) was 10 times larger than those lightly affected (tumor score category 1). Most tumors were found on or near the front and rear flippers; no oral tumors or internal tumors were found. At recapture, 41% of formerly tumor-free turtles revealed FP, often increasing in severity with time, and very few turtles showed signs of disease regression. From the results of this study we concluded that FP is particularly severe in Espírito Santo Bay. Future studies should focus on evaluating how widespread FP is in Brazil, whether prevalence is increasing or decreasing, and elucidating the pathology and pathogenesis of FP in sea turtles in Brazil.