Navegando por Assunto "Microrganismos cavernícolas"

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Artigo
Enzymatic and antimicrobial activity of microorganisms isolated from a cave in the Amazon region
(2023-03-24) Lima, Brenda Almeida; Scherer, Rafael dos Santos ; Albino, Ulisses Brigatto ; Siqueira, Felipe; Bitencourt, José Augusto Pires ; Santos, Sidnei Cerqueira dos
Cave microorganisms, mainly in iron lithology, are poorly known. These constitute most of the cave's biodiversity and play a key role in maintaining the cave ecosystem. The isolation and characterization of the cave microbiota can lead to identifying new species and obtaining substances of biotechnological interest. This work aimed to isolate and evaluate the enzymatic and antimicrobial activity of microorganisms isolated from the cave soil of Parque Nacional dos Campos Ferruginosos National Park, southeastern Amazon. Soil samples were collected from the inner surface layer of cave GEM-1462 in different zones (aphotic, dysphotic and photic). To characterize the strains, the Gram stain technique and biochemical test were used. The microorganisms' proteolytic, cellulolytic, amylolytic, nitrogen fixation, phosphate solubilization, and actinomycete activities were evaluated. Antimicrobial activity was evaluated by antibiosis assay, disk diffusion test and minimal inhibitory concentration against standard bacterial strains. In total, 10 distinct microbial colonies were isolated and all showed enzymatic activity, highlighting the proteolytic activity. Four microbial strains showed antimicrobial activity against Bacillus subtilis and Klebsiella pneumoniae strains. This study demonstrated the first discovery of biological activity of iron cave microorganisms, indicating that the GEM-1462 cave is a promising source for prospecting microorganisms with biotechnological potential, mainly of proteolytic enzymes.