Navegando por Autor "NARO-MACIEL, E"

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Artigo
Deeper mitochondrial sequencing reveals cryptic diversity and structure in Brazilian green turtle rookeries.
(Chelonian Conservation and Biology, 2015) SHAMBLIN, B. M; DUTTON, P. H; BJORNDAL, K. A; BOLTEN, A. B; NARO-MACIEL, E; SANTOS, A. J. B; BELLINI, C; BAPTISTOTTE, C; MARCOVALDI, M. A; NAIRN, C. J
Genetic markers are often used to designate population units for management and conservation, but widespread sharing of mitochondrial DNA control-region haplotypes defined from short (, 500 base-pair [bp]) sequences often limits inferences of population connectivity in marine turtles. Haplotype CM-A8, defined from 490-bp sequences, dominated the haplotype profiles of the 3 major green turtle (Chelonia mydas) rookeries in Brazil. Previous analyses based on 490-bp haplotypes did not detect differentiation between the northern rookeries of Atol das Rocas and Fernando de Norohna, but did indicate differentiation of the northern rookeries from Trindade Island in the south. We reexamined the stock structure of the Brazilian green turtle rookeries using 817-bp control region and mitochondrial short tandem repeat (mtSTR) sequences. Nine 490-bp haplotypes were subdivided into 41 haplotypes by combining 817-bp and mtSTR sequences. Eight of the 14 CM-A8 turtles from Fernando de Noronha carried mtSTR haplotypes that were not detected in the larger rookeries. Pairwise exact tests indicated that the northern Brazilian green turtle rookeries of the Rocas Atoll and Fernando de Noronha are discrete populations with respect to female natal homing. Moreover, several apparently endemic markers in the 3 Brazilian green turtle nesting populations should improve resolution of future mixedstock analyses. Comparable data are needed from green turtle rookeries in the central and eastern Atlantic to assess structure and connectivity at the ocean basin scale.
Produção bibliográfica
Not just another mixed stock analysis: green turtles of Espírito Santo, Brazil.
(2010) NARO-MACIEL, E; MARTIN, M; BONDIOLI, A. C. V; ALMEIDA, A. P; TOREZANI, E; BAPTISTOTTE, C; MARCOVALDI, M. A; AMATO, G; DE SALLE, R
In the Southwestern Atlantic Ocean, sea turtles are exposed to myriad threats including disease, fisheries bycatch, and industrial or coastal development, but protected by effective conservation organizations. In Espirito Santo, Brazil, green turtles (Chelonia mydas) with relatively high incidence of fibropapillomatosis tumors routinely strand in the vicinity of the state capital, Vitória, a highly urbanized area that encompasses the effluent discharge channel of a local steel plant. This is also a particularly interesting population because of its relative proximity to the regionally important Trindade Island rookery, whose feeding grounds have not been convincingly identified to date. To investigate the population distribution of the at-risk turtles, we sequenced a segment of the mitochondrial control region (862 bp; n = 132). Eight mtDNA haplotypes were revealed, of which the most common were CMA-08 and CMA-05. Haplotypes CMA-06 and CMA-09 were each found in six individuals, and rare haplotypes CMA-03, CMA-10, CMA-23, and CMA-32 were also detected. Two kinds of "many-to-many" mixed stock analyses were carried out, taking into account or alternately disregarding source nesting population size. The same approach was taken with traditional MSAS ("one-to-many"), and the main differences between the "one-to-many" and "many-to-many" results are reported. The analyses that included population size and all available data were most consistent with expectations. We recommend caution when employing different mixed stock analysis methods, and emphasize the importance of exploring alternate ways of investigating the origins of mixed stocks, including modeling approaches. These data will provide insight into population isolation and conservation priorities necessary to establish whether areas should be managed as independent units or as regional populations, and will clarify questions of scale in conservation and management, providing a scientific basis for conservation prioritization.
Texto publicado em revista
The Interplay of Homing and Dispersal in Green Turtles: A Focus on the Southwestern Atlantic
(Journal of Heredity, 2012-10-08) NARO-MACIEL, E; BONDIOLI, A.C.V; MARTIN, M; ALMEIDA, A.P; BAPTISTOTTE, C; BELLINI, C; MARCOVALDI, M.A; SANTOS, A.J.B; AMATO, G
Current understanding of spatial ecology is insufficient in many threatened marine species, failing to provide a solid basis for conservation and management. To address this issue for globally endangered green turtles, we investigated their population distribution by sequencing a mitochondrial control region segment from the Rocas Atoll courtship area (n = 30 males) and four feeding grounds (FGs) in Brazil (n = 397), and compared our findings to published data (nnesting = 1205; nfeeding = 1587). At Rocas Atoll, the first Atlantic courtship area sequenced to date, we found males were differentiated from local juveniles but not from nesting females. In combination with tag data, this indicates possible male philopatry. The most common haplotypes detected at the study sites were CMA-08 and CMA-05, and significant temporal variation was not revealed. Although feeding grounds were differentiated overall, intra-regional structure was less pronounced. Ascension was the primary natal source of the study FGs, with Surinam and Trindade as secondary sources. The study clarified the primary connectivity between Trindade and Brazil. Possible linkages to African populations were considered, but there was insufficient resolution to conclusively determine this connection. The distribution of FG haplotype lineages was nonrandom and indicative of regional clustering. The study investigated impacts of population size, geographic distance, ocean currents, and juvenile natal homing on connectivity, addressed calls for increased genetic sampling in the southwestern Atlantic, and provided data important for conservation of globally endangered green turtles.